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JoVE Journal
Immunology and Infection

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Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells
 

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

Article doi: 10.3791/55536
July 3rd, 2017

Summary July 3rd, 2017

We describe a method for the qualitative and quantitative analysis of stress granule formation in mammalian cells after the cells are challenged with bacteria and a number of different stresses. This protocol can be applied to investigate the cellular stress granule response in a wide range of host-bacterial interactions.

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