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JoVE Journal Developmental Biology
En co-kultur metode til at studere Neurite Udvækst som reaktion på Dental Pulp Paracrine Signaler
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En co-kultur metode til at studere Neurite Udvækst som reaktion på Dental Pulp Paracrine Signaler

Article DOI: 10.3791/60809-v • 08:06 min • February 14th, 2020
February 14th, 2020 •
1, 1, 1
1Cell, Developmental and Integrative Biology Department, University of Alabama at Birmingham

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Vi beskriver isolation, spredning og plating af dental pulp (DP) primære celler med trigeminus (TG) neuroner dyrket oven overliggende transwell filtre. Cellulære reaktioner af DP-celler kan analyseres med immunfluorescens eller RNA/proteinanalyse. Immunfluorescens af neuronale markører med konfokal mikroskopi tillader analyse af nitrit udvækst reaktioner.

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Udviklingsmæssige Biologi neurovidenskab udviklingsmæssige biologi tand innervation trigeminusganglion co-kultur metoder
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