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Journal / Biochemistry / Visualizzazione dei recettori degli estrogeni nei …
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Biochemistry

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JoVE Journal Biochemistry
Visualizzazione dei recettori degli estrogeni nei coloni dei topi con la malattia di Crohn indotta da TNBS utilizzando l'immunofluorescenza
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Visualizzazione dei recettori degli estrogeni nei coloni dei topi con la malattia di Crohn indotta da TNBS utilizzando l'immunofluorescenza

Article DOI: 10.3791/60813-v • 08:02 min • March 12th, 2020
March 12th, 2020 •
1, 2, 3, 2, 1
1Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, 2Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, 3Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection, University of Lodz

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Il protocollo presenta un modello murino completo convalidato dalla TNBS della malattia di Crohn e metodi per la visualizzazione dei recettori estrogeni mediante immunosofochimica utilizzando l'immunofluorescenza delle sezioni del colon fissato in formalina incorporate nella paraffina.

Tags

Biochimica Numero 157 Malattia di Crohn modello murino recettori estrogeni recettori estrogeni accoppiati a proteine G GPER ER ER immunoistochimica microscopia confocale
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