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JoVE Journal
Developmental Biology

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使用激光切割模具实现简单的无光刻单细胞微模式
 
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使用激光切割模具实现简单的无光刻单细胞微模式

Article DOI: 10.3791/60888-v 08:59 min April 3rd, 2020
April 3rd, 2020

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Summary

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Click here for the English version.

该协议引入了一种无光刻图的微模式方法,该方法对于生物工程背景有限的人来说简单且易于使用。该方法利用定制的激光切割模具,以感兴趣的形状进行微模式细胞外基质蛋白,以调节细胞形态。微模式程序使用诱导多能干细胞衍生心肌细胞进行演示。

Tags

发育生物学,第158期,单细胞微模式,模具,细胞形态学,无光刻,iPSC-CM,水凝胶
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