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Developmental Biology
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使用单卵母细胞记者测定在体外成熟期间的母体 mRNA 翻译程序
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
Please note that all translations are automatically generated.
Click here for the English version.
使用单卵母细胞记者测定在体外成熟期间的母体 mRNA 翻译程序
DOI:
10.3791/62041-v
•
08:00 min
•
June 16, 2021
•
Natasja G. J. Costermans
2
,
Enrico M. Daldello
2,3
,
Ria J. Marathe
2
,
Marco Conti
2
1
Center for Reproductive Sciences
,
University of California at San Francisco
,
2
Department of Obstetrics Gynecology and Reproductive Sciences
,
University of California at San Francisco
,
3
Present affiliation: Laboratoire de Biologie du Développement-Institut de Biologie Paris Seine, LBD-IBPS
,
Sorbonne Université
Chapters
00:04
Introduction
00:38
Aspiration of Denuded Oocyte and its Microinjection
03:18
Time-lapse Microscopy and Analysis of Ypet-3 UTR Translation
06:00
Results: Statistical Analysis of the Expression for Reporter-YFP and mCherry in Prophase I and Maturing Oocytes
07:19
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了记者在体外成熟期间研究单卵母细胞
mRNA
翻译的调节。
Tags
Maternal MRNA Translation
In Vitro Oocyte Maturation
Single Oocyte Reporter Assay
Time-lapse Microscopy
Reporter Accumulation
Translational Activation
Translational Repression
Antral Follicle
Cumulus-oocyte Complex (COC)
Denuding
Cilostamide
Microinjection
MCherry
Ypet
Time-lapse Imaging
Article
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