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Journal / Biochemistry / 用于活细胞超分辨率显微镜和单分子跟踪的传统 BODIPY…
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Biochemistry

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JoVE Journal Biochemistry
用于活细胞超分辨率显微镜和单分子跟踪的传统 BODIPY 结合物
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用于活细胞超分辨率显微镜和单分子跟踪的传统 BODIPY 结合物

Article DOI: 10.3791/60950-v • 07:49 min • June 8th, 2020
June 8th, 2020 •
1, *1, *1,2, 1
1School of Physics and Astronomy, University of Minnesota, Twin Cities, Physics and Nanotechnology (PAN), 2Middlebury College
* These authors contributed equally

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Summary
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传统的BODIPY结合物可用于活细胞单分子定位显微镜（SMLM），其瞬时成型、红移地面状态变暗器。我们提出了优化的SMLM协议，以跟踪和解决活哺乳动物和酵母细胞中的亚细胞中性脂质和脂肪酸在纳米长度尺度上。

Tags

生物化学， 问题 160， 荧光显微镜， 超分辨率显微镜， 单分子跟踪， 单分子定位显微镜， BODIPY， 地面状态昏暗剂， 酵母， 哺乳动物细胞
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