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Journal / Biochemistry / Konventionelle BODIPY konjugatter til Live-Cell…
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Biochemistry

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JoVE Journal Biochemistry
Konventionelle BODIPY konjugatter til Live-Cell Super-Resolution mikroskopi og Single-Molecule Tracking
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Journal (Biochemistry)
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Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription
Journal (Biochemistry)
Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription
 
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Konventionelle BODIPY konjugatter til Live-Cell Super-Resolution mikroskopi og Single-Molecule Tracking

Article DOI: 10.3791/60950-v • 07:49 min • June 8th, 2020
June 8th, 2020 •
1, *1, *1,2, 1
1School of Physics and Astronomy, University of Minnesota, Twin Cities, Physics and Nanotechnology (PAN), 2Middlebury College
* These authors contributed equally

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Konventionelle BODIPY konjugatter kan bruges til live-celle enkelt-molekyle lokalisering mikroskopi (SMLM) gennem udnyttelse af deres forbigående danner, rød-forskudt jord tilstand dimers. Vi præsenterer en optimeret SMLM protokol til at spore og løse subcellulære neutrale lipider og fedtsyrer i levende pattedyr og gær celler på nanoskopisk længde skala.

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Biokemi Fluorescensmikroskopi Super-opløsning mikroskopi single-molekyle tracking single-molekyle lokalisering mikroskopi BODIPY jord-tilstand dimers gær pattedyr celler
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