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Journal / Biochemistry / Enkeltcelleanalyse af transskriptionsaktive…
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Biochemistry

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JoVE Journal Biochemistry
Enkeltcelleanalyse af transskriptionsaktive alleler efter enkeltmolekyle FISH
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Enkeltcelleanalyse af transskriptionsaktive alleler efter enkeltmolekyle FISH

Article DOI: 10.3791/61680-v • 06:26 min • September 20th, 2020
September 20th, 2020 •
1,2, 1,3, 1,2, 1,2, 1,2,3,4
1GCC Center for Advanced Microscopy and Image Informatics, 2Department of Molecular and Cellular Biology, Baylor College of Medicine, 3Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, 4Department of Pharmacology and Chemical Biology, Baylor College of Medicine

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Enkeltmolekyle RNA fluorescens in situ hybridisering (smFISH) er en metode til præcist kvantificering niveauer og lokalisering af specifikke RNA'er på enkelt celleniveau. Her rapporterer vi vores validerede laboratorieprotokoller til vådbænkbehandling, billedbehandling og billedanalyse til kvantificering af enkeltcellede kvantificering af specifikke RNA'er.

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Biokemi Udgave 163 Enkeltmolekyle FISH billeddannelse billedanalyse gentransskription
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