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Journal / Biochemistry / MeCP2蛋白质变异的电化学发光测定
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Biochemistry

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JoVE Journal Biochemistry
MeCP2蛋白质变异的电化学发光测定
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MeCP2蛋白质变异的电化学发光测定

Article DOI: 10.3791/61054-v • 07:44 min • May 22nd, 2020
May 22nd, 2020 •
1, 1, 1, 2, 3, 1, 1, 1
1Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna (MUV), 2Murdoch Children's Research Institute and Department of Paediatrics, University of Melbourne, Discipline of Child & Adolescent Health, Sydney Medical School, 3Department of Medical Chemistry and Pathobiochemistry, Medical University of Vienna

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电化学发光免疫测定（ECLIA）是一种定量检测内源和外源应用的MeCP2蛋白变异的新方法，在广泛的工作范围内产生高定量、准确和可重复的测量，检测内误差低。此处介绍了 MeCP2-ECLIA 的 96 井格式的协议。

Tags

生物化学， 第 159 期， ECLIA， Sulfo-TAG， MeCP2， TAT 融合蛋白， TAT-MeCP2， 雷特综合征， 蛋白质替代疗法
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