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Journal / Biochemistry / 双色荧光互相关光谱研究活细胞中蛋白质-蛋白质相互作用和蛋白质动力学…
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Biochemistry
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JoVE Journal Biochemistry
双色荧光互相关光谱研究活细胞中蛋白质-蛋白质相互作用和蛋白质动力学
Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
Journal (Biochemistry)
Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
Journal (Biochemistry)
PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
 
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双色荧光互相关光谱研究活细胞中蛋白质-蛋白质相互作用和蛋白质动力学

Article DOI: 10.3791/62954-v • 14:12 min • December 11th, 2021
December 11th, 2021 •
*1, *1, 1, 1, 1, 2, 1
1Rudolf Virchow Center for Integrative and Translation Bioimaging, Julius-Maximilian University Wuerzburg, 2Max Delbrück Center for Molecular Medicine, Berlin
* These authors contributed equally

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我们提出了一种实验方案和数据分析工作流程，以执行活细胞双色荧光交叉相关光谱（FCCS）与Förster共振能量转移（FRET）相结合，以使用现代荧光标记技术研究活细胞中的膜受体动力学。

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生物化学，第178期，
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