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Journal / Biochemistry / MeCP2 Protein Varyantları için…
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Biochemistry

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JoVE Journal Biochemistry
MeCP2 Protein Varyantları için Elektrokemilüminesans Bazlı Bir Test
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MeCP2 Protein Varyantları için Elektrokemilüminesans Bazlı Bir Test

Article DOI: 10.3791/61054-v • 07:44 min • May 22nd, 2020
May 22nd, 2020 •
1, 1, 1, 2, 3, 1, 1, 1
1Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna (MUV), 2Murdoch Children's Research Institute and Department of Paediatrics, University of Melbourne, Discipline of Child & Adolescent Health, Sydney Medical School, 3Department of Medical Chemistry and Pathobiochemistry, Medical University of Vienna

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Elektrokemilüminesans immünoassay (ECLIA), geniş bir çalışma aralığında düşük intra ve inter-assay hatası ile yüksek nicel, doğru ve tekrarlanabilir ölçümler üreten endojen ve eksojen olarak uygulanan MeCP2 protein varyantlarının kantitatif tespiti için yeni bir yaklaşımdır. Burada, 96-iyi formatta MeCP2-ECLIA için protokol açıklanmıştır.

Tags

Biyokimya Sayı 159 ECLIA Sulfo-TAG MeCP2 TAT füzyon proteini TAT-MeCP2 Rett sendromu protein replasmanı tedavisi
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