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P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives
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Immunology and Infection
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JoVE Journal Immunology and Infection
P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives

P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives

DOI:
10.3791/61069-v

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10:26 min

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June 15, 2020

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, , , , , , , ,
1Department of Drug Delivery,Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), 2Department of Pharmacy,Saarland University, 3Department of Vaccinology and Applied Microbiology,Helmholtz Centre for Infection Research, 4Department of Internal Medicine V – Pulmonology, Allergology, Respiratory Intensive Care Medicine,Saarland University Hospital

Chapters

  • 00:04Introduction
  • 01:26Growth and Differentiation of Cells in Permeable Support Inserts
  • 05:29Infection by P. aeruginosa
  • 07:00Measurement of Bacterial Proliferation via Colony-forming Units (CFU)
  • 08:00Results: Epithelial-macrophage Co-cultures Infected with P. aeruginosa
  • 09:48Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol for a three-dimensional co-culture model of infected airways, using CFBE41o- cells, THP-1 macrophages, and Pseudomonas aeruginosa, established at the air-liquid interface. This model provides a new platform to simultaneously test antibiotic efficacy, epithelial barrier function, and inflammatory markers.

Tags

Keywords: P. Aeruginosa3D Co-cultureBronchial Epithelial CellsMacrophagesAir-liquid InterfaceAnti-infectivesBiofilmsHost CellsEpithelial BarriersLung InfectionAerosol AdministrationHuman CellsInfection ResearchCell CultivationCFBE41 O Minus CellsCell SeedingPermeable Supports

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