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Multicellulær human alveolær model sammensat af epitelceller og primære immunceller til farevurdering
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Multicellulær human alveolær model sammensat af epitelceller og primære immunceller til farevurdering

Article DOI: 10.3791/61090-v • 09:27 min • May 6th, 2020
May 6th, 2020 •
*1, *1, 1, 1
1Adolphe Merkle Institute, University of Fribourg
* These authors contributed equally

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Præsenteret her er en protokol for primær human blod monocyt isolation samt deres differentiering i makrofager og dendritiske celler og samling med epitelceller i en multicellulær menneskelige lunge model. Biologiske reaktioner af cocultures bestående af immunceller differentieret fra enten frisk isolerede eller optøede monocytter, ved udsættelse for proinflammatoriske stimuli, sammenlignes.

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Biologi human lunge cocultures 3D menneskelige cokulturer luft-flydende interface isolering af menneskelige perifere blod monocytter primære makrofager og dendritiske celler kryoreserveret primære immunceller
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