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JoVE Journal
Biology

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由上皮细胞和原发免疫细胞组成的多细胞人类Alveal模型,用于危险评估
 
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由上皮细胞和原发免疫细胞组成的多细胞人类Alveal模型,用于危险评估

Article DOI: 10.3791/61090-v 09:27 min May 6th, 2020
May 6th, 2020

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

这里介绍的是原发性人类血液单细胞分离的协议,以及它们分化成巨噬细胞和树突状细胞,并组装上皮细胞成多细胞人类肺模型。比较了由免疫细胞组成的生物反应,这些细胞与新鲜分离或解冻的单核细胞不同,在接触刺激性刺激时。

Tags

生物学,第159期,人类肺共培养,3D人类共培养,空气-液体界面,人类周围血单核分离,原发巨噬细胞和树突状细胞,冷冻保存原生免疫细胞
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