Here, we detail how to synchronize Drosophila to a circadian day. This is the first, and most important step necessary for studying biological rhythms and chronobiology.
Dada, A. O., Nguyen, M. Q., Peterson, S. M., Ngo, V. T., Cornelio-Parra, D. V., Omer, B. S., Thapa, A., Rapp, S. R., Cloud, V. J., Mohan, R. D. Circadian Entrainment of Drosophila Melanogaster. J. Vis. Exp. (160), e61176, doi:10.3791/61176 (2020).