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Immunology and Infection

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JoVE Journal Immunology and Infection
Identifiering och karakterisering av immunogenic RNA Arter i HDM Allergener som modulerar Eosinofil lunginflammation
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Identifiering och karakterisering av immunogenic RNA Arter i HDM Allergener som modulerar Eosinofil lunginflammation

Article DOI: 10.3791/61183-v • 08:44 min • May 30th, 2020
May 30th, 2020 •
1,2, 1,3, 1
1Department of Microbiology, Immunology and Molecular Genetics, Long School of Medicine, University of Texas Health San Antonio, 2Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, 3Department of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University

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Miljöallergener som dammkvalster (HDM) innehåller ofta mikrobiella ämnen som aktiverar medfödda immunsvar för att reglera allergisk inflammation. Protokollet presenteras här visar identifiering av DSRNA arter i HDM allergener och karakterisering av deras immunogenic verksamhet i modulering eosinofila lungcancer inflammation.

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Immunologi och infektion BAL bronchoalveolar lavage dsRNA FACS fluorescensaktiverad cellsortering HDM hus dammkvalster ISGs interferon stimulerade gener PAMP patogen-associerade molekylära mönster RT-qPCR
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