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Identifikation og karakterisering af immunogeneRNA-arter i HDM-allergener, der modulerer eosinofil lungebetændelse
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1Department of Microbiology, Immunology and Molecular Genetics, Long School of Medicine,University of Texas Health San Antonio, 2Department of Clinical Laboratory Sciences, College of Applied Medical Sciences,Jouf University, 3Department of Otolaryngology-Head and Neck Surgery,Xiangya Hospital, Central South University
Chapters
- 00:04Introduction
- 01:02Dot Blot to Show the Presence of dsRNA Structures in HDM Total RNA
- 03:54FACS Analysis to Determine the Effects of HDM RNA on the Infiltration of Eosinophils in BAL and Lung
- 06:19Results: dsRNA Structures in HDM RNAs
- 07:53Conclusion
Miljømæssige allergener såsom husstøvmider (HDM) indeholder ofte mikrobielle stoffer, der aktiverer medfødte immunreaktioner for at regulere allergisk inflammation. Den protokol, der præsenteres her, viser identifikationen af dsRNA-arter i HDM-allergener og karakterisering af deres immunogene aktiviteter i modulerende eosinofil lungebetændelse.
