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Journal / Cancer Research / Opprette matchede in vivo/in vitro…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Opprette matchede in vivo/in vitro pasientavledede modellpar av PDX- og PDX-avledede organoider for kreftfarmakologiforskning
A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
Journal (Cancer Research)
A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Journal (Cancer Research)
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
 
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Opprette matchede in vivo/in vitro pasientavledede modellpar av PDX- og PDX-avledede organoider for kreftfarmakologiforskning

Article DOI: 10.3791/61382-v • 04:49 min • May 5th, 2021
May 5th, 2021 •
1, 1, 2, 2, 2, 1, 2, 2, 1, 2, 3, 2, 2, 2, 3
1Crown Bioscience Inc., Changping District, Beijing, China, 2Crown Bioscience Inc., Taicang, Jiangsu, China, 3Crown Bioscience Inc., San Diego, CA, USA

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En metode er beskrevet for å lage organoider ved hjelp av pasientavledede xenografts (PDX) for in vitro screening, noe som resulterer i matchede par in vivo / in vitro-modeller. PDX-svulster ble høstet/behandlet i små biter mekanisk eller enzymatisk, etterfulgt av Clevers' metode for å dyrke tumororganoider som ble passasjert, kryopreservert og karakterisert mot den opprinnelige PDX.

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Denne måneden i JoVE Utgave 171 In vitro kreft modell dyr tumor modell morfologi histopatologi genomisk profilering kjemoterapi
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