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JoVE Journal
Immunology and Infection

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人类自然杀手细胞代谢分析
 
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人类自然杀手细胞代谢分析

Article DOI: 10.3791/61466-v 09:03 min June 22nd, 2020
June 22nd, 2020

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Summary

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Click here for the English version.

在这张纸上,我们描述了一种测量从周围血液分离的原生人类自然杀手(NK)细胞中的糖解和线粒体呼吸的方法,在休息时或IL15诱导的活化之后。所述协议可以很容易地扩展到由其他细胞因子或可溶性刺激激活的原发性人类NK细胞。

Tags

免疫学和感染, 问题 160, 自然杀手细胞, 新陈代谢, 细胞外通量, 糖解, 线粒体, 呼吸, 电子传输链, 流细胞
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