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An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood
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Immunology and Infection
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JoVE Journal Immunology and Infection
An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

DOI:
10.3791/55615-v

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07:21 min

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June 23, 2017

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, , , , ,
1Institute of Applied Technology, Hefei Institutes of Physical Science,Chinese Academy of Sciences, 2University of Science and Technology of China, 3Department of Physics and Astronomy,University of Manitoba, 4Department of Biosystems Engineering,University of Manitoba, 5Seven Oaks General Hospital, 6Department of Immunology,University of Manitoba, 7Department of Biological Sciences,University of Manitoba

Chapters

  • 00:05Title
  • 00:46Microfluidic Device Fabrication
  • 03:06Microfluidic Cell Migration Assay Preparation
  • 03:52On-chip Cell Isolation and Chemotaxis Assay
  • 05:46Results: All-on-chip Neutrophil Chemotaxis Response to fMLP Chemical Gradient
  • 06:51Conclusion

Summary

Automatic Translation

Automatic Translation

This article provides the detailed method of performing a rapid neutrophil chemotaxis assay by integrating the on-chip neutrophil isolation from whole blood and the chemotaxis test on a single microfluidic chip.

Tags

Keywords: Neutrophil ChemotaxisMicrofluidic DeviceWhole BloodNeutrophil SeparationCell MigrationChemotaxis AssayPDMSSU8 MoldPlasma TreatmentFibronectin

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