An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

Ke Yang; Jiandong Wu; Ling Zhu; Yong Liu; Michael Zhang; Francis Lin

doi:10.3791/55615

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

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07:21 min

June 23, 2017

DOI: 10.3791/55615-v

Ke Yang*^1,2,3, Jiandong Wu*^3,4, Ling Zhu¹, Yong Liu¹, Michael Zhang⁵, Francis Lin^3,4,6,7

¹Institute of Applied Technology, Hefei Institutes of Physical Science,Chinese Academy of Sciences, ²University of Science and Technology of China, ³Department of Physics and Astronomy,University of Manitoba, ⁴Department of Biosystems Engineering,University of Manitoba, ⁵Seven Oaks General Hospital, ⁶Department of Immunology,University of Manitoba, ⁷Department of Biological Sciences,University of Manitoba

This article provides the detailed method of performing a rapid neutrophil chemotaxis assay by integrating the on-chip neutrophil isolation from whole blood and the chemotaxis test on a single microfluidic chip.

The overall goal of this procedure is to demonstrate a method to separate neutrophils from a drop of whole blood and then perform a subsequent chemotaxis assay using a single microfluidic device. This method provides our code to perform a neutrophil chemotaxis assay using small volume of whole blood. And can be easily applied by researchers to answer biological questions related to immune cell migration.

The main advantage of this technique is integration of and our chemotaxis assay on single microfluidic device. This permits assembled and analysis in 25 minutes. To begin, prepare the master maldus described in the accompanying text protocol.

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