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Intratracheal Administration of Dry Powder Formulation in Mice
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JoVE Journal Medicine
Intratracheal Administration of Dry Powder Formulation in Mice

Intratracheal Administration of Dry Powder Formulation in Mice

DOI:
10.3791/61469-v

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07:55 min

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July 25, 2020

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, , ,
1Department of Pharmacology and Pharmacy, LKS Faculty of Medicine,The University of Hong Kong, 2Advanced Drug Delivery Group, Sydney Pharmacy School, Faculty of Medicine and Health,The University of Sydney

Chapters

  • 00:04Introduction
  • 01:22Fabrication of Dry Powder Insufflator and Loading of Dry Powder
  • 02:36Intubation
  • 04:17Powder Administration
  • 05:25Results: Optimization of Intratracheal Administration of mRNA Formulation
  • 07:23Conclusion

Summary

Automatic Translation

Automatic Translation

Dry powder formulations for inhalation have great potential in treating respiratory diseases. Before entering human studies, it is necessary to evaluate the efficacy of the dry powder formulation in preclinical studies. A simple and noninvasive method of the administration of dry powder in mice through the intratracheal route is presented.

Tags

Intratracheal AdministrationDry Powder FormulationMiceInsufflatorPulmonary AbsorptionBioavailabilityTherapeutic EffectsPre-clinical Animal ModelsIntubationStatic ChargesGel Loading Pipette TipWeighing PaperSyringeAnesthesiaBALB/c MouseOptical FiberGuiding Cannula

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