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使用
循环介质等温扩
增检测灌溉水中的植物浮肿
JoVE Journal
Environment
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JoVE Journal
Environment
Detection of
Phytophthora capsici
in Irrigation Water using Loop-Mediated Isothermal Amplification
Please note that all translations are automatically generated.
Click here for the English version.
使用
循环介质等温扩
增检测灌溉水中的植物浮肿
DOI:
10.3791/61478-v
•
07:25 min
•
June 25, 2020
•
Owen Hudson
,
Sumyya Waliullah
,
Justin Hand
,
Romina Gazis-Seregina
,
Fulya Baysal-Gurel
,
Md Emran Ali
1
Department of Plant Pathology, Coastal Plain Experiment Station
,
University of Georgia
,
2
University of Georgia Cooperative Extension
,
3
Department of Plant Pathology, Tropical Research & Education Center
,
University of Florida
,
4
Department of Agricultural and Environmental Sciences, Otis L. Floyd Nursery Research Center
,
Tennessee State University
Chapters
00:04
Introduction
00:48
Pump and Filter Setup
01:16
Water Sampling and Filtering
02:22
Magnetic Bead-Based DNA Extraction
03:58
Loop-Mediated Isothermal Amplification (LAMP) Assay
05:07
Results: Representative
Phytophthora capsici
Detection
06:53
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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italiano (Italian)
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português (Portuguese)
русский (Russian)
español (Spanish)
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Automatic Translation
我们开发了一种
利用
滤纸DNA提取方法检测水源中植物性浮肿的方法,并结合循环中等温扩增(LAMP)测定法,可现场或实验室进行分析。
Tags
Phytophthora Capsici
Irrigation Water
Loop-mediated Isothermal Amplification
Pathogen Detection
DNA Extraction
Filter Paper
Magnetic Beads
Irrigation Water Contamination
Waterborne Pathogen
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