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JoVE Journal Biochemistry
Estrategia fosfoproteómica para perfilar la señalización de estrés osmótico en Arabidopsis
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Estrategia fosfoproteómica para perfilar la señalización de estrés osmótico en Arabidopsis

Article DOI: 10.3791/61489-v • 05:47 min • June 25th, 2020
June 25th, 2020 •
1,3, 2, 3,4, 5
1Department of Plant Biology, Carnegie Institute for Science, 2Graduate School of Pharmaceutical Sciences, Kyoto University, 3Department of Biochemistry, Purdue University, 4Department of Chemistry, Purdue University, 5Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences

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Aquí se presenta un enfoque fosfoproteómico, a saber, detener e ir extracción a base de punta fosfoproteómica, que proporciona alto rendimiento y cobertura profunda de arabidopsis fosfoproteoma. Este enfoque delinea la visión general de la señalización de estrés osmótico en Arabidopsis.

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Bioquímica Número 160 Espectrometría de masas fosfoproteómica fosforilación proteica enfoque de consejos escénicos Etiquetado de etiqueta de masa tándem Arabidopsis,estrés osmótico
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