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JoVE Journal Biochemistry
Phosphoproteomic Strategie zur Profilierung osmotischer Stresssignalisierung bei Arabidopsis
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Phosphoproteomic Strategie zur Profilierung osmotischer Stresssignalisierung bei Arabidopsis

Article DOI: 10.3791/61489-v • 05:47 min • June 25th, 2020
June 25th, 2020 •
1,3, 2, 3,4, 5
1Department of Plant Biology, Carnegie Institute for Science, 2Graduate School of Pharmaceutical Sciences, Kyoto University, 3Department of Biochemistry, Purdue University, 4Department of Chemistry, Purdue University, 5Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences

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Präsentiert wird hier ein phosphoprotemischer Ansatz, nämlich Stop-and-Go-Extraktionsspitze Phosphoproteomic, die hohen Durchsatz und tiefe Abdeckung von Arabidopsis phosphoproteome bietet. Dieser Ansatz beschreibt den Überblick über osmotische Stresssignale in Arabidopsis.

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Biochemie Ausgabe 160 Massenspektrometrie Phosphoproteomik Proteinphosphorylierung Stufenspitzenansatz Tandem-Massen-Tag-Etikettierung Arabidopsis,osmotischer Stress
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