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JoVE Journal Developmental Biology
Un modello Xenotranest derivato dal paziente per la malformazione Venosa
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Un modello Xenotranest derivato dal paziente per la malformazione Venosa

Article DOI: 10.3791/61501-v • 06:51 min • June 15th, 2020
June 15th, 2020 •
*1, *1, 1,2
1Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine
* These authors contributed equally

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Presentiamo un protocollo dettagliato per generare un modello murine xenograft di malformazione venosa. Questo modello si basa sull'iniezione sottocutanea di cellule endoteliali derivate dal paziente contenenti mutazioni genetiche TIE2 e/o PIK3CA iper-attivanti. Le lesioni dello Xenotrapianto ricapitolano da vicino le caratteristiche istopatologiche del tessuto del paziente VM.

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Biologia dello sviluppo Problema 160 Malformazione Venosa xenotrapianto cellule endoteliali TIE2 PIK3CA lesione vascolare
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