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Journal
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Immunology and Infection
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マウスペイヤーパッチからの胚芽センターB細胞のJH4イントロンにおける体細胞ハイパー突然変異の解析
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer’s Patches
Please note that all translations are automatically generated.
Click here for the English version.
マウスペイヤーパッチからの胚芽センターB細胞のJH4イントロンにおける体細胞ハイパー突然変異の解析
DOI:
10.3791/61551-v
•
09:35 min
•
April 20, 2021
•
Emily Sible
,
Simin Zheng
,
Jee Eun Choi
,
Bao Q. Vuong
1
Department of Biology
,
The City College of New York and The Graduate Center of The City University of New York
,
2
Department of Microbiology
,
Icahn School of Medicine at Mount Sinai
Chapters
00:05
Introduction
01:03
Dissection of Peyer’s Patches
02:25
Cell Isolation and Staining for FACS
05:12
JH4 Intron Sequence Amplification and Analysis
07:39
Results: Flow Cytometry and JH4 Sequence Analysis
08:52
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここで提示されるアッセイは、マウスペイヤーのパッチから胚中心B細胞を用いた免疫グロブリン重鎖遺伝子遺伝子軌跡内の体細胞過突然変異を定量化するアッセイである。
Tags
Somatic Hypermutation
JH4 Intron
Germinal Center B Cells
Peyer’s Patches
Antibody Diversification
Molecular Biology
PCR
Sanger Sequencing
Cell Isolation
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