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Immunology and Infection
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从小鼠佩耶的补丁中对Germinal中心B细胞JH4因特龙的体细胞突变分析
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer’s Patches
Please note that all translations are automatically generated.
Click here for the English version.
从小鼠佩耶的补丁中对Germinal中心B细胞JH4因特龙的体细胞突变分析
DOI:
10.3791/61551-v
•
09:35 min
•
April 20, 2021
•
Emily Sible
,
Simin Zheng
,
Jee Eun Choi
,
Bao Q. Vuong
1
Department of Biology
,
The City College of New York and The Graduate Center of The City University of New York
,
2
Department of Microbiology
,
Icahn School of Medicine at Mount Sinai
Chapters
00:05
Introduction
01:03
Dissection of Peyer’s Patches
02:25
Cell Isolation and Staining for FACS
05:12
JH4 Intron Sequence Amplification and Analysis
07:39
Results: Flow Cytometry and JH4 Sequence Analysis
08:52
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里介绍的是一个检测,以量化免疫球蛋白重链基因轨迹内的体细胞突变使用细菌中心B细胞从小鼠Peyer的补丁。
Tags
Somatic Hypermutation
JH4 Intron
Germinal Center B Cells
Peyer’s Patches
Antibody Diversification
Molecular Biology
PCR
Sanger Sequencing
Cell Isolation
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