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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Propagation of the Microsporidian Parasite Edhazardia aedis in Aedes aegypti Mosquitoes
Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation
Journal (Immunology and Infection)
Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation
Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model
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Propagation of the Microsporidian Parasite Edhazardia aedis in Aedes aegypti Mosquitoes

Article DOI: 10.3791/61574-v • 05:29 min • August 13th, 2020
August 13th, 2020 •
*1, *1, 2, 2, 1
1Department of Entomology, The Ohio State University, 2USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology
* These authors contributed equally

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Summary
Automatic Translation

A protocol to culture the microsporidian parasite Edhazardia aedis. The parasite is passaged from one generation of Aedes aegypti mosquitoes to the next via horizontal transfer at the larval stage followed by vertical transmission at the adult stage. Live sporoplasms survive long-term in infected eggs.

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Microsporidian Parasite Edhazardia Aedis Aedes Aegypti Mosquitoes Rearing Protocol Infectious Spores Next Generation Propagation Timing Visual Aids Larval Rearing Tray Fish Food Temperature Humidity Light Cycle Density Of Larvae Cat Food Instars Uninfected A.aegypti Eggs
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