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Journal / Bioengineering / Kontrolleret stamme af 3D Hydrogels under Live…
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JoVE Journal Bioengineering
Kontrolleret stamme af 3D Hydrogels under Live Mikroskop imaging
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Kontrolleret stamme af 3D Hydrogels under Live Mikroskop imaging

Article DOI: 10.3791/61671-v • 07:41 min • December 4th, 2020
December 4th, 2020 •
1, 2, 3, 3, 2, 3,4
1Department of Biomedical Engineering, Faculty of Engineering, Tel-Aviv University, 2Department of Materials Science and Engineering, Faculty of Engineering, Tel-Aviv University, 3School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University, 4Center for the Physics and Chemistry of Living Systems, Tel-Aviv University

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Den præsenterede metode indebærer uniaxial strækning af 3D bløde hydrogels indlejret i silikone gummi og samtidig tillade levende confocal mikroskopi. Karakterisering af de eksterne og interne hydrogelstammer samt fiberjustering demonstreres. Den udviklede enhed og protokol kan vurdere cellernes reaktion på forskellige stammeregimer.

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Bioengineering Hydrogel Extracellular matrix Mekanobiologi Cellemekanik Mekanisk kraft Ekstern strækning Fiber netværk Fiber tilpasning
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