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High-Resolution Imaging af nuklear dynamik i levende celler under Uniaxial træk stamme
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain
DOI:

09:20 min

June 02, 2019

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Chapters

  • 00:04Title
  • 00:37Fabrication of Single-well Polydimethylsiloxane Plates and Square Compartments
  • 02:52Cell Culture
  • 04:28Imaging
  • 07:14Results: Fluctuations Data of the Nucleus in Response to Chemical and Strain Cues
  • 08:31Conclusion

Summary

Automatic Translation

Ved hjælp af en tidligere designet enhed til at anvende mekanisk belastning til vedklædnings celler, dette papir beskriver en redesignet substrat geometri og et tilpasset apparat til høj opløsning enkelt celle billedbehandling af anstrengte celler med en 100x olie fordybelse mål.

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