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High-Resolution Imaging af nuklear dynamik i levende celler under Uniaxial træk stamme
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Bioengineering
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JoVE Journal Bioengineering
High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

High-Resolution Imaging af nuklear dynamik i levende celler under Uniaxial træk stamme

DOI:
10.3791/59474-v

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09:20 min

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June 02, 2019

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, ,
1BioSystems and Micromechanics Interdisciplinary Research Group,Singapore-MIT Alliance for Research and Technology, CREATE, 2Department of Material Science and Engineering,Massachusetts Institute of Technology, 3Department of Biological Engineering,Massachusetts Institute of Technology

Chapters

  • 00:04Title
  • 00:37Fabrication of Single-well Polydimethylsiloxane Plates and Square Compartments
  • 02:52Cell Culture
  • 04:28Imaging
  • 07:14Results: Fluctuations Data of the Nucleus in Response to Chemical and Strain Cues
  • 08:31Conclusion

Summary

Automatic Translation

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Ved hjælp af en tidligere designet enhed til at anvende mekanisk belastning til vedklædnings celler, dette papir beskriver en redesignet substrat geometri og et tilpasset apparat til høj opløsning enkelt celle billedbehandling af anstrengte celler med en 100x olie fordybelse mål.

Tags

High-resolution ImagingNuclear DynamicsLive CellsUniaxial Tensile StrainCell-stretching SystemPolydimethylsiloxaneSubstratum FabricationProgenitor CellsProliferation Medium

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