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Identifisering, isolasjon og karakterisering av Fibro-Adipogenic Progenitors (FAPs) og Myogenic Progenitors (MPs) i skjelettmuskulaturen i rotten
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Identifisering, isolasjon og karakterisering av Fibro-Adipogenic Progenitors (FAPs) og Myogenic Progenitors (MPs) i skjelettmuskulaturen i rotten

Article DOI: 10.3791/61750-v • 09:49 min • June 9th, 2021
June 9th, 2021 •
*1, *1, 1, 1,2
1Keenan Research Center for Biomedical Science, St Michaels Hospital, Unity Health Toronto, 2Department of Medicine and Interdepartmental Division of Critical Care Medicine, University of Toronto
* These authors contributed equally

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Denne protokollen skisserer en metode for å isolere Fibro-adipogene forfedre (FAPer) og myogene forfedre (MPs) fra rotte skjelettmuskulatur. Utnyttelse av rotten i muskelskademodeller gir økt vevstilgjengelighet fra atrofisk muskel for analysen og et større repertoar av validerte metoder for å vurdere muskelstyrke og gang i fritt bevegelige dyr.

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Biologi Utgave 172 mesenchymale forfedre fibro-adipogene forfedre myogene forfedre strømningscytometri fluorescensaktivert cellesortering FACS skjelettmuskulatur kronisk traumatisk denervasjon rotte muskelatrofi fibrose
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