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Developmental Biology

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JoVE Journal Developmental Biology
Dissezione assistita da transilluminazione di stadi specifici del ciclo epiteliale seminifero del topo per analisi immunosottenifere a valle
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Dissezione assistita da transilluminazione di stadi specifici del ciclo epiteliale seminifero del topo per analisi immunosottenifere a valle

Article DOI: 10.3791/61800-v • 09:59 min • October 7th, 2020
October 7th, 2020 •
1, 1, 1, 1, 3,4, 1,2, 3,4, 1, 1
1Institute of Biomedicine, Integrative Physiology and Pharmacology Unit, University of Turku, Turku, Finland, 2Department of Pediatrics, Turku University Hospital, Turku, Finland, 3Centre for Reproductive Health, Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia, 4Australian Regenerative Medicine Institute, Monash University, Melbourne, VIC 3800, Australia

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Questo protocollo descrive la microdissezione assistita dalla transilluminazione di segmenti di tubuli seminiferi di topi adulti che rappresentano stadi specifici del ciclo epiteliale seminifero, e i tipi di cellule in esso, e la successiva immunostaining di preparati di zucca e segmenti di tubuli intatti.

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Biologia dello sviluppo Numero 164 topo spermatogenesi epitelio seminifero stadio transilluminazione preparazione zucca colorazione a montaggio intero spermiogenesi
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