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Developmental Biology
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下流免疫染色解析のためのマウス半離層上皮周期の特定段階のトランスイルミネーション支援解離
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses
Please note that all translations are automatically generated.
Click here for the English version.
下流免疫染色解析のためのマウス半離層上皮周期の特定段階のトランスイルミネーション支援解離
DOI:
10.3791/61800-v
•
09:59 min
•
October 07, 2020
•
Juho-Antti Mäkelä
,
Sheyla Cisneros-Montalvo
,
Tiina Lehtiniemi
,
Opeyemi Olotu
,
Hue M. La
4
,
Jorma Toppari
2
,
Robin M. Hobbs
4
,
Martti Parvinen
,
Noora Kotaja
1
Institute of Biomedicine, Integrative Physiology and Pharmacology Unit
,
University of Turku, Turku, Finland
,
2
Department of Pediatrics
,
Turku University Hospital, Turku, Finland
,
3
Centre for Reproductive Health
,
Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia
,
4
Australian Regenerative Medicine Institute
,
Monash University, Melbourne, VIC 3800, Australia
Chapters
00:04
Introduction
00:51
Testis Dissection and Preparation
02:10
Transillumination-Assisted Microdissection
02:47
Squash Preparation and Stage Verification
04:04
Whole-Mount Seminiferous Tubule Immunostaining
07:04
Results: Representative Mouse Seminiferous Epithelial Cycle Staging
09:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本プロトコルは、半日本円上皮周期の特定段階を表す成体マウス半細管の断面微小切除法、およびその中の細胞タイプ、および後にスカッシュ製剤および無傷の管状セグメントの免疫染色を記述する。
Tags
Transillumination
Seminiferous Epithelial Cycle
Spermatogenesis
Mouse Testes
Dissection
Immunostaining
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