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Genomteknik af primære humane B-celler ved hjælp af CRISPR/Cas9
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Genomteknik af primære humane B-celler ved hjælp af CRISPR/Cas9

Article DOI: 10.3791/61855-v • 08:20 min • November 3rd, 2020
November 3rd, 2020 •
1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3
1Department of Pediatrics, University of Minnesota, 2Center for Genomic Engineering, University of Minnesota, 3Masonic Cancer Center, University of Minnesota

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Her leverer vi en detaljeret, trin-for-trin-protokol til CRISPR/Cas9-baseret genomteknik af primære humane B-celler til gen knockout (KO) og knock-in (KI) til undersøgelse af biologiske funktioner af gener i B-celler og udvikling af B-celleterapi.

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Biologi CRISPR/Cas9 genomteknik rekombinant AAV genredigering primære menneskelige B-celler
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