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Isolation, kultur og genteknologi af pattedyr primære pigment epitelceller til ikke-viral genterapi
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Isolation, kultur og genteknologi af pattedyr primære pigment epitelceller til ikke-viral genterapi

Article DOI: 10.3791/62145-v • 09:46 min • February 26th, 2021
February 26th, 2021 •
*1,2, *1,2, 1,2, 1, 3, 4, 1,2
1Experimental Ophthalmology, University of Geneva, 2Department of Ophthalmology, University Hospitals of Geneva, 3Department of Ophthalmology, University Hospital RWTH Aachen, 4Max Delbrück Center for Molecular Medicine
* These authors contributed equally

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Her præsenteres en protokol om at isolere og transfekte primær iris og retinale pigment epitelceller fra forskellige pattedyr (mus, rotte, kanin, gris og kvæg). Metoden er velegnet til at studere okulære genterapimetoder i forskellige set-ups til ex vivo-analyser og in vivo-studier, der kan overføres til mennesker.

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Biologi okulær genterapi retinal degeneration RPE celler IPE celler celle isolation Tornerose transposon ikke-viral genterapi PEDF GM-CSF
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