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Journal / Neuroscience / Long-Term, Serum-Free Cultivation of Organotypic…
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Neuroscience

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JoVE Journal Neuroscience
Long-Term, Serum-Free Cultivation of Organotypic Mouse Retina Explants with Intact Retinal Pigment Epithelium
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Long-Term, Serum-Free Cultivation of Organotypic Mouse Retina Explants with Intact Retinal Pigment Epithelium

Article DOI: 10.3791/61868-v • 05:52 min • November 25th, 2020
November 25th, 2020 •
*1, *1, 1, 1, 1, 1
1Institute for Ophthalmic Research, University of Tübingen
* These authors contributed equally

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The protocol describes organotypic explants of mouse neuroretina, cultivated together with its retinal pigment epithelium (RPE), in R16 defined medium, free of serum and antibiotics. This method is relatively simple to perform, less expensive, and time-consuming when compared to in vivo experiments, and can be adapted to numerous experimental applications.

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Long-term Serum-free Cultivation Organotypic Mouse Retina Explants Intact Retinal Pigment Epithelium Isolation Technique Normal Development Tissue Architecture Morphology Controlled Conditions Antibiotics-free Serum-free Retinal Diseases Retinitis Pigmentosa Diabetic Retinopathy Pre-clinical Development Novel Treatments Soumaya Belhadj Arianna Tolone PhD Students Incubation Process BM (buffered Saline Solution) Proteinase K Fetal Calf Serum Dissection Process
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