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Journal / Biochemistry / Derivatisering av proteinkrystaller med I3C ved…
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Biochemistry
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JoVE Journal Biochemistry
Derivatisering av proteinkrystaller med I3C ved hjelp av tilfeldig mikroseede matrix screening
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Derivatisering av proteinkrystaller med I3C ved hjelp av tilfeldig mikroseede matrix screening

Article DOI: 10.3791/61894-v • 14:04 min • January 16th, 2021
January 16th, 2021 •
*1, *1,2, 1,2, 1
1School of Biological Sciences, The University of Adelaide, 2Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences, The University of Adelaide
* These authors contributed equally

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Denne artikkelen presenterer en metode for å generere proteinkrystaller derivatisert med I3C (5-amino-2,4,6-triiodoisoftalsyre) ved hjelp av mikroseeding for å generere nye krystalliseringsforhold i sparsomme matriseskjermer. Brettene kan settes opp ved hjelp av flytende dispenseringsroboter eller for hånd.

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Biokjemi Utgave 167 seeding tilfeldig mikrofrømatrisescreening rMMS proteinkrystallografi innfasing Magic Triangle I3C
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