Simple, Affordable, and Modular Patterning of Cells using DNA

Katelyn  A. Cabral; David  M. Patterson; Olivia  J. Scheideler; Russell Cole; Adam  R. Abate; David  V. Schaffer; Lydia  L. Sohn; Zev J. Gartner

doi:10.3791/61937

Journal

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Bioengineering

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Modellazione semplice, economica e modulare delle cellule utilizzando il DNA

Simple, Affordable, and Modular Patterning of Cells using DNA

JoVE Journal
Bioengineering

This content is Free Access.

JoVE Journal Bioengineering

Simple, Affordable, and Modular Patterning of Cells using DNA

Modellazione semplice, economica e modulare delle cellule utilizzando il DNA

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4,099 Views

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08:59 min

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February 24, 2021

DOI:

10.3791/61937-v

DOI:

10.3791/61937-v

•

08:59 min

February 24, 2021

•

4064 Views

Katelyn A. Cabral¹, David M. Patterson², Olivia J. Scheideler¹, Russell Cole³, Adam R. Abate^4,5,6, David V. Schaffer^7,8, Lydia L. Sohn⁹, Zev J. Gartner^2,6,10

¹Graduate Program in Bioengineering,University of California San Francisco and University of California Berkeley ²Department of Pharmaceutical Chemistry,University of California San Francisco ³Scribe Biosciences ⁴Department of Bioengineering and Therapeutic Sciences,University of California San Francisco ⁵California Institute for Quantitative Biosciences,University of California San Francisco ⁶Chan Zuckerberg Biohub,University of California San Francisco ⁷Department of Chemical & Biomolecular Engineering,University of California Berkeley ⁸Helen Wills Neuroscience Institute,University of California Berkeley ⁹Department of Mechanical Engineering,University of California Berkeley ¹⁰Center for Cellular Construction,University of California San Francisco

Summary

Qui presentiamo un protocollo alle cellule micropattern a risoluzione monocellulare utilizzando l'adesione programmata dal DNA. Questo protocollo utilizza una piattaforma di fotolitografia da banco per creare modelli di oligonucleotidi del DNA su un vetrino e quindi etichetta le membrane cellulari con oligonucleotidi complementari disponibili in commercio. L'ibridazione degli oligos si traduce in adesione cellulare_programmata.