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Journal / Neuroscience / Imagem transpupilar de dois fótons in vivo da…
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Neuroscience

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JoVE Journal Neuroscience
Imagem transpupilar de dois fótons in vivo da retina do rato
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Imagem transpupilar de dois fótons in vivo da retina do rato

Article DOI: 10.3791/61970-v • 09:03 min • February 13th, 2021
February 13th, 2021 •
1,2, 1,2, 1,3,4
1John F. Hardesty, MD Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, 2Graduate Program in Neuroscience, Washington University School of Medicine, 3Department of Neuroscience, Washington University School of Medicine, 4Hope Center for Neurological Disorders, Washington University School of Medicine

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A imagem in vivo é uma ferramenta poderosa para o estudo da biologia na saúde e na doença. Este protocolo descreve imagens transpupilares da retina de camundongos com um microscópio padrão de dois fótons. Também demonstra diferentes métodos de imagem in vivo para marcar fluorescentemente múltiplas coortes celulares da retina.

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Neurociência Edição 168 retina imagens in vivo microscopia de dois fótons células ganglionares da retina células amácrinas micróglia
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