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Estimering av krystallinsk celluloseinnhold av plantebiomasse ved hjelp av Updegraff-metoden
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Estimation of Crystalline Cellulose Content of Plant Biomass using the Updegraff Method
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12:34 min

May 15, 2021

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Chapters

  • 00:00Introduction
  • 01:12Phase I: Preparation of Samples
  • 02:02Phase II: Preparation of the Alcohol Insoluble Crude Cell Wall
  • 04:52Phase III: Removal of Hemicellulose and Lignin using Updegraff Reagent
  • 07:10Phase IV: Acid Hydrolysis of Cellulose into Glucose Monomers
  • 07:49Phase V: Anthrone Assay to Estimate the Glucose Concentration of Samples and Glucose Standard
  • 10:04Representative Results & Conclusion

Summary

Automatic Translation

Updegraff-metoden er den mest brukte metoden for celluloseestimering. Hovedformålet med denne demonstrasjonen er å gi en detaljert Updegraff-protokoll for estimering av celluloseinnhold i plantebiomasseprøver.

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