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Análise da tradução no cérebro do rato em desenvolvimento usando perfil polino

Article DOI: 10.3791/62088-v • 08:38 min • May 22nd, 2021
May 22nd, 2021 •
1,3, 2,3, 1,2,3
1Department of Biochemistry and Molecular Biology; Cumming School of Medicine, University of Calgary, 2Department of Medical Genetics, Cumming School of Medicine, University of Calgary, 3Alberta Children's Hospital Research Institute, University of Calgary

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O desenvolvimento do cérebro mamífero requer o controle adequado da expressão genética no nível da tradução. Aqui, descrevemos um sistema de perfil polisso com uma plataforma de gradiente e fracionamento de sacarose fácil de montar para avaliar o status translacional de mRNAs no cérebro em desenvolvimento.

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Neurociência Edição 171
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