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Isolatie, kweek en genetische manipulatie van primaire pigmentepitheelcellen van zoogdieren voor niet-virale gentherapie
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Isolatie, kweek en genetische manipulatie van primaire pigmentepitheelcellen van zoogdieren voor niet-virale gentherapie

Article DOI: 10.3791/62145-v • 09:46 min • February 26th, 2021
February 26th, 2021 •
*1,2, *1,2, 1,2, 1, 3, 4, 1,2
1Experimental Ophthalmology, University of Geneva, 2Department of Ophthalmology, University Hospitals of Geneva, 3Department of Ophthalmology, University Hospital RWTH Aachen, 4Max Delbrück Center for Molecular Medicine
* These authors contributed equally

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Hier wordt een protocol gepresenteerd om primaire iris- en retinale pigmentepitheelcellen van verschillende zoogdieren (muizen, ratten, konijnen, varkens en runderen) te isoleren en te transfecteren. De methode is bij uitstek geschikt om oculaire gentherapiebenaderingen te bestuderen in verschillende opstellingen voor ex vivo analyses en in vivo studies die overdraagbaar zijn op mensen.

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Biologie Nummer 168 oculaire gentherapie retinale degeneratie RPE-cellen IPE-cellen celisolatie Doornroosje transposon niet-virale gentherapie PEDF GM-CSF
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