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Journal / Immunology and Infection / Hochdurchsatz-Transkriptomanalyse zur…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Hochdurchsatz-Transkriptomanalyse zur Untersuchung von Wirt-Pathogen-Interaktionen
Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster
Journal (Immunology and Infection)
Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster
Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment
Journal (Immunology and Infection)
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Hochdurchsatz-Transkriptomanalyse zur Untersuchung von Wirt-Pathogen-Interaktionen

Article DOI: 10.3791/62324-v • 14:58 min • March 5th, 2022
March 5th, 2022 •
1,2, 3, 2,3, 3, 2,3, 2,3,4
1Laboratory of Pathology of Infectious Diseases, Department of Pathology, Medical School, University of São Paulo, 2Scientific Platform Pasteur USP, 3Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, 4Hospital Israelita Albert Einstein

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Das hier vorgestellte Protokoll beschreibt eine komplette Pipeline zur Analyse von RNA-Sequenzierungs-Transkriptomdaten von Rohlesungen bis hin zur Funktionsanalyse, einschließlich Qualitätskontroll- und Vorverarbeitungsschritten bis hin zu fortschrittlichen statistischen Analyseansätzen.

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Immunologie und Infektion Ausgabe 181 Hochdurchsatzsequenzierung RNA-seq Wirt-Pathogen-Interaktion differentiell exprimierte Gene koexprimierte Gene Funktionsanalyse molekularer Störungsgrad von Proben
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