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Antivirale analyser med høy gjennomstrømning til skjerm for hemmere av Zika-virusreplikering
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication
DOI:

10:16 min

October 30, 2021

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Chapters

  • 00:02Introduction
  • 01:44Luciferase Activity Assay
  • 03:53Cell Proliferation-Based MTT Assay
  • 04:27NS2B-NS3 Protease Activity Assay
  • 05:19NS5 RdRp Elongation Assay
  • 06:32Representative Results
  • 08:06Conclusions

Summary

Automatic Translation

I dette arbeidet beskriver vi protokollene som brukes i replikonbaserte og virale enzymbaserte analyser for å screene for hemmere av Zika-virusreplikasjon i et screeningformat med høy gjennomstrømning.

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