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吸收荧光标记的小细胞外囊泡 在体外 和脊髓

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

Please note that all translations are automatically generated. Click here for the English version.

吸收荧光标记的小细胞外囊泡在体外和脊髓

DOI:

10.3791/62537-v

•

09:01 min

•

May 23, 2021

•

Richa Gupta, Xuan Luo, Zhucheng Lin, Yuzhen Tian, Seena K. Ajit

¹Department of Pharmacology & Physiology,Drexel University College of Medicine

Chapters

00:04Introduction
00:57Uptake of sEVs by Neuro-2a Cells
01:38Primary Astrocytic Cultures
03:34Immunofluorescence
05:03In Vivo Uptake of sEVs and Immunohistochemistry
06:43Results: Uptake of Fluorescent Labeled Small Extracellular Vesicles
08:11Conclusion

Summary

Automatic Translation

我们描述了一个协议，用PKH染料标记大噬细胞衍生的小细胞外囊泡，并在体外和脊髓中观察它们 在体外 和脊髓内分娩后的吸收情况。

Tags

Keywords: Fluorescent Labeled Small Extracellular Vesicles SEVs Uptake Spinal Cord In Vitro Cell Visualization Neuro-2a Cells Postnatal Pups Cortical Lobes Dissociation Astrocytes Microglia Oligodendrocytes Mechanistic Studies Therapeutic Studies Spinal Disorders Pain Inflammation

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