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Neuroscience
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吸收荧光标记的小细胞外囊泡
在体外
和脊髓
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Uptake of Fluorescent Labeled Small Extracellular Vesicles
In Vitro
and in Spinal Cord
Please note that all translations are automatically generated.
Click here for the English version.
吸收荧光标记的小细胞外囊泡
在体外
和脊髓
DOI:
10.3791/62537-v
•
09:01 min
•
May 23, 2021
•
Richa Gupta
,
Xuan Luo
,
Zhucheng Lin
,
Yuzhen Tian
,
Seena K. Ajit
1
Department of Pharmacology & Physiology
,
Drexel University College of Medicine
Chapters
00:04
Introduction
00:57
Uptake of sEVs by Neuro-2a Cells
01:38
Primary Astrocytic Cultures
03:34
Immunofluorescence
05:03
In Vivo Uptake of sEVs and Immunohistochemistry
06:43
Results: Uptake of Fluorescent Labeled Small Extracellular Vesicles
08:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个协议,用PKH染料标记大噬细胞衍生的小细胞外囊泡,并在体外和脊髓中观察它们
在体外
和脊髓内分娩后的吸收情况。
Tags
Fluorescent Labeled Small Extracellular Vesicles
SEVs Uptake
Spinal Cord
In Vitro
Cell Visualization
Neuro-2a Cells
Postnatal Pups
Cortical Lobes
Dissociation
Astrocytes
Microglia
Oligodendrocytes
Mechanistic Studies
Therapeutic Studies
Spinal Disorders
Pain
Inflammation
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