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JoVE Journal Cancer Research
En Ex Vivo Brain Slice Model til at studere og målrette brystkræft hjernemetastatisk tumorvækst
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En Ex Vivo Brain Slice Model til at studere og målrette brystkræft hjernemetastatisk tumorvækst

Article DOI: 10.3791/62617-v • 10:38 min • September 22nd, 2021
September 22nd, 2021 •
1, 1, 1, 1, 2,4, 3,4, 2, 1,4
1Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, 2Department of Pharmacology and Physiology, Drexel University College of Medicine, 3Department of Radiation Oncology, Thomas Jefferson University, 4Sidney Kimmel Cancer Center, Thomas Jefferson University

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Vi introducerer en protokol til måling af realtids lægemiddel- og strålingsrespons af brystkræft hjernemetastatiske celler i en organotypisk hjerneskivemodel. Metoderne giver et kvantitativt assay til at undersøge de terapeutiske virkninger af forskellige behandlinger på hjernemetastaser fra brystkræft på en ex vivo-måde inden for hjernens mikromiljøgrænseflade.

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Kræftforskning udgave 175 ex vivo-model hjernetumor intrakraniel tumorinjektion hjernemetastase af brystkræft
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