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An Ex Vivo Brain Slice Model to Study and Target Breast Cancer Brain Metastatic Tumor Growth

Article DOI: 10.3791/62617-v • 10:38 min • September 22nd, 2021
September 22nd, 2021 •
1, 1, 1, 1, 2,4, 3,4, 2, 1,4
1Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, 2Department of Pharmacology and Physiology, Drexel University College of Medicine, 3Department of Radiation Oncology, Thomas Jefferson University, 4Sidney Kimmel Cancer Center, Thomas Jefferson University

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We introduce a protocol for measuring real-time drug and radiation response of breast cancer brain metastatic cells in an organotypic brain slice model. The methods provide a quantitative assay to investigate the therapeutic effects of various treatments on brain metastases from breast cancer in an ex vivo manner within the brain microenvironment interface.

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Ex Vivo Brain Slice Model Breast Cancer Brain Metastasis Tumor Growth Drug Efficacy Radiation Testing Brain-tumor-host Interactions Brain Metastatic Cells Brain Tumor Microenvironment Cancer Growth And Spread Procedure Demonstration Lorela Ciraku Emily Esquea PhD Candidates Stereotaxic Frame Head Immobilization Iodine Swabs Ethanol Sterilization Surgical Scalpel Midline Incision Skull Exposure Injection Site GFP Luciferase
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