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Journal / Cancer Research / İnsan Periferik Kan Lenfositlerinde γ-H2AX Odak…
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Cancer Research
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JoVE Journal Cancer Research
İnsan Periferik Kan Lenfositlerinde γ-H2AX Odak Analizi için Otomatik Mikroskobik Puanlama Yöntemi
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İnsan Periferik Kan Lenfositlerinde γ-H2AX Odak Analizi için Otomatik Mikroskobik Puanlama Yöntemi

Article DOI: 10.3791/62623-v • 08:23 min • December 25th, 2021
December 25th, 2021 •
1, 2, 1, 3, 1, 1, 1, 1, 4, 5, 6, 1
1Radiation Biophysics Division, Nuclear Medicine Department, iThemba LABS, 2Department of Biotechnology, University of the Western Cape, 3Department of Medical Biosciences, University of the Western Cape, 4Division of Medical Physiology, Department of Medicine and Health Sciences, Stellenbosch University, 5Biodosimetry Service, Instituto de Investigaciones Biológicas Clemente Estable and Academic Unit on Radiation Protection, Faculty of Medicine, University of the Republic, 6MetaSystems Hard & Software GmbH

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Bu protokol, periferik kan lenfositleri üzerinde γ-H2AX odak analizinin slayt hazırlanmasını ve otomatik puanlamasını sunar. Tahlil yöntemini ve hassasiyetini göstermek için, izole lenfositler in vitroışınlandı. Dna DSB algılamanın bu otomatik yöntemi, hızlı ve yüksek verimli biyolojik dozimetri uygulamaları için yararlıdır.

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