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Visualisering af membranflæsedannelse ved hjælp af scanningselektronmikroskopi
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Visualisering af membranflæsedannelse ved hjælp af scanningselektronmikroskopi

Article DOI: 10.3791/62658-v • 08:05 min • May 27th, 2021
May 27th, 2021 •
1, 1, 2, 1,3
1Vascular Biology Center, Medical College of Georgia at Augusta University, 2Department of Cellular Biology and Anatomy, Medical College of Georgia at Augusta University, 3Department of Pharmacology and Toxicology, Medical College of Georgia at Augusta University

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Makropinocytose er en stærkt bevaret endocytisk proces initieret ved dannelsen af F-actinrige arklignende membranfremspring, også kendt som membranflæser. Øget hastighed af makropinocytotisk solind internalisering har været impliceret i forskellige patologiske tilstande. Denne protokol præsenterer en metode til at kvantificere membranflæsedannelse in vitro ved hjælp af scanningselektronmikroskopi.

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