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Journal / Cancer Research / Flow Cytometric Analysis of Apoptotic Biomarkers…
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Cancer Research

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JoVE Journal Cancer Research
Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
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Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

Article DOI: 10.3791/62663-v • 13:53 min • August 26th, 2021
August 26th, 2021 •
1,2, 1, 1
1Department of Chemical Pathology, Faculty of Health Sciences, University of Pretoria, 2Tshwane Academic Division, National Health Laboratory Service

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Summary
Automatic Translation

Apoptosis can be characterized by flow cytometric analysis of early and late apoptotic biomarkers. The cervical cancer cell line, SiHa, was analyzed for apoptosis biomarkers after treatment with Actinomycin D using a benchtop flow cytometer.

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Flow Cytometric Analysis Apoptotic Biomarkers Actinomycin D-treated SiHa Cervical Cancer Cells Multiparametric Analysis Single Cells Biomarker Identification Cell Staining Non-expert Flow Cytometrists Cell Culture Cell Detachment Trypsinization Cell Seeding Attachment Assay Actinomycin D Treatment
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